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β-胡萝卜素的液液萃取

Liquid Liquid Extraction of β-carotene

VERSA液液萃取工作站对β-胡萝卜素液相萃取(分配色谱法)的验证

Validation of Automated Liquid-Liquid Extraction (Partition Chromotography) of β-Carotene Using VERSA Mini Liquid-Liquid Extraction Workstation

液液萃取,或分配色谱,可以是一个手动密集和耗时的程序,为许多下游应用。Aurora Biomed验证了VERSA 110液-液萃取工作站自动执行这些程序。在该验证中,用水-乙醇(1:1)样品加入850µg/mL的β-胡萝卜素。工作站被编程为使用甲板上的振动筛或通过液体处理头的移液管作用自动混合样品(液相)和己烷溶剂(有机相)。混合后,提取有机相。提取曲线显示,在第一次和第二次提取中,使用振动筛分别提取了90.1%和9.3%的β-胡萝卜素。振动台提供了更好的分配相移液管。此外,还比较了两种不同转速(1100rpm和700rpm)下的萃取曲线,发现1100rpm的振荡有更好的分离效果。

Liquid liquid extraction, or partition chromotography, can be a manually intensive and time-consuming procedure for many downstream applications. Aurora Biomed validated the VERSA Mini (110) Liquid-Liquid Extraction workstation to automatically perform these procedures. In this validation, the water-alcohol (1:1) sample was spiked with beta-carotene at 850µg/mL. The workstation was programmed to either auto-mix the sample (liquid phase) and hexane solvent (organic phase) using a shaker on the deck or by pipette action of the liquid-handling head. After mixing, the organic phase was extracted. The extraction profile showed that 90.1 and 9.3% of the beta-carotene was extracted in the first and second extraction, respectively, using the deck shaker. The deck shaker provided better partitioning of the phases that the pipette. In addition, the extraction profiles at two different shaker speeds (1100rpm and 700rpm) were compared and it was found that 1100rpm shaking resulted in better partitioning.

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