ICR 8000 初级药物筛选技术
Aurora Biomed的离子通道阅读器（ICR）将原子吸收的多功能性、精密度和灵敏度与我们获得专利的微进样工艺和液体处理技术相结合，为离子通道研究人员创造了高通量的筛选解决方案，填补了自动化膜片钳无法填补的空白。ICR8000是一种更经济实惠的、中等吞吐量的离子通道筛选系统，对于台式机来说足够紧凑，同时保留了广泛的实用性。
ICR 8000可用于评估电压门控（hERG、BK/SK、Kv1.1、1.4、1.5、KCNQ、2P等）和配体门控（KATP、nAChR等）离子通道，以及离子泵和转运体（如Na+/K+-ATP酶），使研究人员能够加速药物开发，用于治疗和预防与这些关键膜转运蛋白相关的疾病。
目前的应用包括阳离子氯共转运蛋白（CCC）的评估，CCC是神经元氯稳态的关键组成部分，是细胞体积和神经元兴奋性的重要调节因子。因为CCC是电中性的，它们的活性不能用自动膜片钳技术来测量。ICR 8000能够通过测量细胞溶质和细胞外离子的绝对浓度来获得这些和其他通道及转运蛋白的高通量活性测量，这一技术独立于依赖于电压操纵的方法，并且是对这些方法的补充。
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ICR 8000 离子通道阅读器
规格
- 通量：高达5000孔每天
- 单通道：一次测量一个样本
- 最小样本体积：50µl
- 载体：96/384微孔板
- 体积：高67 cm X 宽55 cm X 深37 cm
- 燃料来源：乙炔/压缩空气
- 选配：板材堆垛机/条形码阅读器
- 灵敏度：0.05 ppm检测限
- 精度:＜5%CV
特点&优势
- 高达5000孔每天，可编程及自动化的解决方案
- 自动稀释，校准和清洗
- 适用于常规自动化
- 不需要样品稀释
- 高灵敏度
- 在线稀释
- 消除与荧光相关的猝灭效应
- 无需使用危险的放射性同位素
- 避免Rb半衰期短造成的工作限制
- 非常适合hERG通道分析
- 可评估电致和电中性转运体
常见问题
非放射性通量分析与膜片钳相比如何？
How does the non-radioactive flux assay compare to patch clamp?
非放射性通量分析被认为是一个高通量的解决方案，以研究广泛的膜蛋白，包括电中性目标，传统的电生理学无法应用。通量分析能够建立与膜片钳相同的药物效力等级。The non-radioactive flux assay is considered a high throughput solution to investigate a broad range of membrane proteins including electro-neutral targets, to which conventional electrophysiology cannot be applied. The flux assay is capable of establishing the same drug potency rank orders as patch clamps.
ICR可以应用于什么类型的膜蛋白靶点？
What type of membrane protein targets can the ICR be applied to?
膜蛋白靶点包括电压门控和配体门控离子通道、共转运蛋白和泵。Membrane protein targets including voltage-gated and ligand-gated ion channels, co-transporters and pumps.
建立通量分析的一般程序是什么？
What is the general procedure of setting up flux assays?
表达感兴趣的离子通道的细胞在被送入ICR之前要经过一系列的程序，包括示踪剂装载、药物孵育、清洗、处理和裂解。ICR可以完全自动化样品分析程序Cells expressing the ion channels of interest go through a protocol of tracer loading, drug incubation, wash, treatment, and lysis before they are be fed into the ICR. The ICR can fully automate the sample analysis procedure.
ICR 8000和ICR 12000的主要区别是什么？
What are the main differences between the ICR 8000 and ICR 12000?
ICR 8000的最小样品装载量为50 ul，ICR 12000的最小样品装载量为20 ul。ICR 12000每天处理60000孔，而ICR 8000每天处理5000孔。ICR 12000还有一个可选的叠盘机。The minimal sample loading volume is for 50 ul for the ICR 8000 and 20 ul for the ICR 12000. The ICR 12000 has a throughput of processing 60000 wells/day as compared to 5000 wells/day on the ICR 8000. The ICR 12000 also has an optional plate stacker.
什么酶标板与ICR兼容？
What plate formats are compatible with the ICR?
标准96和384孔板。Standard 96 and 384 well plates.
使用ICR的用户有哪些？
Who are some of the organizations using the ICR?
我们的客户包括Amgen, Merck, AstraZeneca, Roche, Pfizer, 不列颠哥伦比亚大学、河北医科大学等。Our customers include Amgen, Merck, AstraZeneca, Roche, Pfizer, University of British Columbia, Heibei Medical University, and more.
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Applications
Publications
- • Nonradioactive Rubidium Efflux Assay Technology for Screening of Ion Channels（Georg C. Terstappen）
- • Ion Flux and Ligand Binding Assays for Analysis of Ion Channels（Georg C. Terstappen）
- • Development and validation of HTS assay for screening the calcium-activated chloride channel modulators in TMEM16A stably expressed CHO cells
- • Screening technologies for ion channel drug discovery
- • Analogs of MK-499 are differentially affected by a mutation in the S6 domain of the hERG K+ channel
- • High-throughput analysis of drug binding interactions for the human cardiac channel, Kv1.5
- • Cellular HTS Assays for Pharmacological Characterization of Na V 1.7 Modulators
- • Zinc pyrithione-mediated activation of voltage-gated KCNQ potassium channels rescues epileptogenic mutants
- • Evaluation of the Rubidium Efflux Assay for Preclinical Identification of hERG Blockade
- • Characterization of a hERG Screen Using the IonWorks HT: Comparison to a hERG Rubidium Efflux Screen
- • Development of an HTS Assay for Na , K -ATPase Using Nonradioactive Rubidium Ion Uptake
- • Rb + Flux through hERG Channels Affects the Potency of Channel Blocking Drugs: Correlation with Data Obtained Using a High-Throughput Rb + Efflux Assay
- • Atomic Absorption Spectroscopy in Ion Channel Screening
- • Nonradioactive Rubidium Ion Efflux Assay and Its Applications in Drug Discovery and Development
- • Validation of an Atomic Absorption Rubidium Ion Efflux Assay for KCNQ/M-Channels Using the Ion Channel Reader 8000
- • High Throughput Assay Technologies for Ion Channel Drug Discovery
- • A High Throughput Screening Techno logy-Overcoming Bottlenecks in Ion Channel Drug Targets
- • A medium-throughput functional assay of KCNQ2 potassium channels using rubidium efflux and atomic absorption spectrometry